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Key Documents

GP2d

95090714, human colon, Epithelial

別名:

GP2d Cells

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About This Item

UNSPSCコード:
41106514

product name

GP2d, 95090714

由来生物

human colon

成長モード

Adherent

核型

Diploid

形態

Epithelial

製品

Not specified

受容体

Epidermal growth factor (EGF)

テクニック

cell culture | mammalian: suitable

関連疾患

cancer

輸送温度

dry ice

保管温度

−196°C

細胞株の由来

Human Caucasian colon adenocarcinoma

細胞株の説明

GP2d has been established from the same adenocarcinoma as GP5d (Sigma Catalogue number. 95090715). This has been confirmed by STR profiling at ECACC. The cells were derived from a local recurrence of Duke′s grade B, poorly differentiated carcinoma of the colon from a 71 year old female at surgical resection. The patient received no preoperative chemo- or radiotherapy and had a strong family history of colon cancer with two first degree relatives dying of the disease below the age of 55 years. Both clones possess genetic changes consistent with the pattern of tumour progression in colon cancer but are morphologically distinct. An interstitial deletion on chromosome 5 (region 14q to 22q) and an inverted duplication of bands 10q11 and 10q21 has been reported. Both cell lines contain a normal copy of the ki-ras gene and a copy with a transition in codon 12. GP2d cells show growth response to EGF, TGF α or insulin with an increase in cell numbers. Amphiregulin mRNA is abundant in GP2d but almost undetectable in GP5d.

アプリケーション

GP2d has been used to extract genomic DNA for digital PCR (dPCR). It has also been used to extract DNA from the cell with PIK3CA mutation c.3140A>T.

DNAプロファイル

STR-PCR Data: Amelogenin: X
CSF1PO: 9,12
D13S317: 8,12
D16S539: 11,13
D5S818: 11,12
D7S820: 9,11
THO1: 7,9.3
TPOX: 8,11
vWA: 17,18

培地

DMEM + 2mM グルタミン + 10% ウシ胎児血清(FBS)

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 5x10,000 to 1x100,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

その他情報

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