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製品名
Hepa-1c1c7, 95090613
由来生物
mouse liver
成長モード
Adherent
核型
Not specified
形態
Epithelial
製品
Not specified
受容体
Aryl hydrocarbon
テクニック
cell culture | mammalian: suitable
関連疾患
cancer
輸送温度
dry ice
保管温度
−196°C
細胞株の由来
Mouse hepatoma
細胞株の説明
Hepa-1c1c7 was cloned from the cell line Hepa-1cl which was obtained from Hepa-1. Hepa-1 was derived from the BW7756 hepatoma that developed in a C57L mouse. Hepa-1c1c7 cells express the aryl hydrocarbon (Ah) receptor and were shown to be highly inducible for cytochrome P450IA1. A variety of mutants from this cell line have been established.
アプリケーション
Study of Ah receptors, P450IA1 regulation
培地
α MEM without nucleosides + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
継代と培養方法
Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
その他情報
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