RIN-m

95071701, rat pancreas, Epithelial-like

• UNSPSCコード: 41106514

特性

• 製品名: RIN-m, 95071701
• 由来生物: rat pancreas
• 成長モード: Adherent
• 核型: Not specified
• 形態: Epithelial-like
• 製品: Somatostatin, insulin
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Rat islet cell tumour

細胞株の説明

Established from a transplantable pancreatic tumour of the islets of Langerhans. The tumour originated in an irradiated NEDH inhibited albino rat and was maintained by serial transplantation in Nedh rats. After transplantation into athymic nude mice the cell line RIN-m was established from the fourth nude mouse heterotransplant, 9 days after the tumour was removed cells were exposed to cystine-free medium resulting in the complete elimination of fibroblast-like cells. RIN-m cells secrete insulin and somatostatin. High levels of L-dopa-decarboxylase and membrane bound secretory granules were detected. RIN-5F (95090402) was established as a secondary clone from RIN-m.

アプリケーション

Study of biology of pancreatic islet cells, synthesis, storage and secretion of insulin and somatostatin

培地

RPMI 1640 + 2mM グルタミン + 10％ ウシ胎児血清（FBS）

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

その他情報

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