PG

95070629, fish ovary, Fibroblast-like

• UNSPSCコード: 41106514

特性

• product name: PG, 95070629
• 由来生物: fish ovary
• 成長モード: Adherent
• 核型: Aneuploid
• 形態: Fibroblast-like
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Fish Northern Pike ovaries

細胞株の説明

The fish cell line PG has been initiated 1976 from ovarian tissue of 5 juvenile pike (Esox lucius). Cells grow at 4°C to 25°C with an optimum temperature of 20°C. PG cells are susceptible to various fish viruses including infectious pancreatic necosis viruses and pike fry rhabdovirus. The cell line has been mycoplasma eradicated at ECACC.

アプリケーション

Fish Virology

培地

MEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 20°C Fish cell lines detach easily during transit if the culture is too young. After resuscitation split cells 1:3 to 1:4 and culture for at least one week wit

その他情報

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