PNT2 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 95012613, human prostate, Epithelial

• 別名: PNT-2 Cells
• UNSPSCコード: 41106514

特性

• 由来生物: human prostate
• 包装: tube of 5 μg 95012613-DNA-5UG; pkg of vial of cells 95012613-1VL
• 成長モード: Adherent
• 核型: Not specified
• 形態: Epithelial
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human prostate normal, immortalised with SV40

細胞株の説明

Established by immortalisation of normal adult prostatic epithelial cells by transfection with a plasmid containing SV40 genome with a defective replication origin. The primary culture was obtained from a prostate of a 33 year old male at post mortem. PNT2 cells contain the SV40 genome and express large T protein. They possess a well differentiated morphology with the expression of cytokeratin 8,18 and 19 with the latter being a feature of differentiated luminal cells of the glandular prostate. Cytokeratin 14, a marker of epithelial basal cells, is not expressed. The cells are non-tumourigenic in nude mice.

アプリケーション

Biology of prostate cells, model of differentiated prostate epithelial cells.

PNT2 cell line has been used:

• to assess the effects of Verapamil, an L-type calcium channel blocker on autophagy
• to assess the cytotoxicity of aqueous dilutions of a universal self-priming dental adhesive (DA)
• to study the expression of soluble adenylyl cyclase (sAC) in the cells

DNAプロファイル

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,13
D13S317: 8,13
D16S539: 9
D5S818: 12,13
D7S820: 11
THO1: 6,9.3
TPOX: 8,9
vWA: 17

培地

RPMI 1640 + 2mM Glutamine + 6 -10% Foetal Bovine Serum (FBS).

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

その他情報

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