SK-N-BE(2)

95011815, human nerve, Epitheilioid & neuroblastoid

• UNSPSCコード: 41106514

特性

• product name: SK-N-BE(2), 95011815
• 由来生物: human nerve
• 成長モード: Adherent
• 核型: Modal no. 44
• 形態: Epitheilioid & neuroblastoid
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: metastasis
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human Caucasian neuroblastoma

細胞株の説明

Established from a bone marrow biopsy of a 22-month-old male with disseminated neuroblastoma in 1972. The morphology varies with sub-population from cells with long processes through small, flattened, epithelioid cells. Dense cultures tend to form loosely adherent aggregates. They are multipotential with regard to neuronal enzyme expression and display a high capacity to convert tyrosine to dopamine.

アプリケーション

Chemotherapy development, chromosome studies, drug resistance, differentiation studies.

培地

EMEM (EBSS) + 1% Non Essential Amino Acids (NEAA) : Ham′s F12 (1:1) + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

継代と培養方法

Split sub-confluent cultures (70-80%) 1:10 to 1:25 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

その他情報

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