AT6.1

94101452, rat prostate, Epithelial

• UNSPSCコード: 41106514

特性

• product name: AT6.1, 94101452
• 由来生物: rat prostate
• 成長モード: Adherent
• 核型: 44XY, +4, + 12 del(3)(3q32-36)del(15)p14
• 形態: Epithelial
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Rat dorsal prostatic adenocarcinoma

細胞株の説明

A highly metastatic, anaplastic, androgen-independent sub-line which spontaneously developed during the serial passage of the non-metastatic, well-differentiated, androgen-responsive R-3327-H sub-lines in the laboratory of Dr J Schalken, Nijmegan, The Netherlands.

アプリケーション

Used in studies in which micro-cell mediated chromosomal transfer of human chromosome 11 suppressed metastatic ability. This suppresser function was localised to 11p11.2-13.

培地

RPMI 1640 + 2mM Glutamine + 250nM Dexamethasone (DXMT) + 10% Foetal Bovine Serum (FBS).

継代と培養方法

Split sub-confluent cultures (70-80%) 1:10 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

その他情報

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