VA-ES-BJ

94092303, human skin (bone marrow metastasis), Epithelial

• UNSPSCコード: 41106514

特性

• product name: VA-ES-BJ, 94092303
• 由来生物: human skin (bone marrow metastasis)
• 成長モード: Adherent
• 核型: 66-68 Aneuploidic, pentasomy #7, multiple trisomy
• 形態: Epithelial
• 製品: Granulocyte Macrophage Colony Stimulating Factor (GM-CSF)
• 受容体: Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), AE, AE3, Fas, epithelial membrane antigen (EMA), Vimentin, P53
• テクニック: cell culture | mammalian: suitable
• 関連疾患: metastasis
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human skin epithelioid sarcoma

細胞株の説明

Spontaneously transformed cells from a male patient with epithelioid sarcoma derived from bone marrow metastasis. Cells grow very rapidly and secrete GM-CSF. They have been shown to express AE-1 and AE-3 cytokeratins, epithelial membrane antigen, p53, human Fas and vimentin. VA-ES-BJ cells are sensitive to oxidising agents, busulfaxamine and protoporphyrin IX. Epithelial, spindle and giant cells.

アプリケーション

Study of tumours. Tumourigenic in nude mice.

培地

DMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

その他情報

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