AtT-20/D16v-F2

94050406, mouse brain, Epithelial

• UNSPSCコード: 41106514

特性

• 製品名: AtT-20/D16v-F2, 94050406
• 由来生物: mouse brain
• 包装: tube of 5 μg 94050406-DNA-5UG; pkg of vial of cells 94050406-1VL
• 成長モード: Adherent
• 核型: Not specified
• 形態: Epithelial
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Mouse pituitary tumour

細胞株の説明

This F2 subclone was developed from original AtT-20, an andrenocorticotropic hormone (ACTH) secreting cell line established from murine pituitary tumour. These cells are readily transfected using a standard calcium phosphate protocol.

アプリケーション

AtT-20/D16v-F2 has been used to study the role of the G-protein Rasd1 in hypothalamic response to neuronal activation. It has also been used to study the effect of cAMP and glucocorticoid treatment on cAMP-responsive element binding protein 3 like 1 (CREB3L1).

培地

DMEM + 2mM Glutamine + 10% FBS.

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 to 1:5 using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Cell clumps must be broken up to prevent cells growing in suspension.

その他情報

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