EoL-1 cell

94042252, human blood, Lymphoblast

• 別名: AML-EOL-1 Cells, EoL1 Cells, Eosinophilic Leukemia Cell Line
• UNSPSCコード: 41106514

特性

• product name: EoL-1 cell, 94042252
• 由来生物: human blood
• 成長モード: Suspension
• 核型: Not specified
• 形態: Lymphoblast
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human eosinophilic leukaemia

細胞株の説明

Differentiated by N-butylate treatment.

アプリケーション

EoL-1 cell line has been used to study the role of AGS67E, an antibody-drug conjugate that targets cluster of differentiation-37 (CD37) for the treatment of B/T-cell malignancies. It has also been used to study the role of anti-vascular cell adhesion molecule-1 (VCAM-1) monoclonal antibody in inhibiting asthma in a murine asthma model.

DNAプロファイル

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 7,10
D13S317: 12,13
D16S539: 8,10
D5S818: 11
D7S820: 10,13
THO1: 9.3
TPOX: 8
vWA: 16,17

培地

RPMI 1640 + 2mM グルタミン + 10％ ウシ胎児血清（FBS）

継代と培養方法

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. Eol-1 cells grow very slowly and may take up to 5 days to reach a suitable level to subculture. It is best that these cells are left to grow and not agitated more than necessary.The cells actually grow as large clusters. They are beginning to reach confluence when the cells in the centre of each cluster start to look unhealthy. The change in media colour should also be an indication. Eol-1 cells prefer slightly acidic conditions and so may resuscitate better from frozen if started up in 15% foetal bovine serum.

その他情報

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