NFS-25 C-3

93090713, mouse blood, Lymphoblast

• UNSPSCコード: 41106514

特性

• product name: NFS-25 C-3, 93090713
• 由来生物: mouse blood
• 成長モード: Suspension
• 核型: Not specified
• 形態: Lymphoblast
• 製品: Not specified
• 受容体: Ly-17, Lyb-2, Ly-1, Ly-5 (B220)
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Mouse pre-B lymphoblast

細胞株の説明

Derived from a spontaneous lymphoma in an NFS.C58v-1 mouse. These pre-B cells are negative for surface Ig, Ia and ThB.

培地

DMEM + 10% NCTC 109 + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1% Sodium Pyruvate (NaP) + 1mM Oxaloacetic acid + 50μM 2-Mercaptoethanol (2ME) + 2μg/ml Transferrin + 0.2IU/ml Insulin + 10% Foetal Bovine Serum (FBS).

継代と培養方法

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. Immediately after resuscitation a pre-centrifugation step to remove cryoprotectant (DMSO) is recommended i.e. pellet the cells by centrifugation at 100 x g for 5 minutes and resuspend the cell

その他情報

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