MEWO

93082609, human skin, Fibroblast

• UNSPSCコード: 41106514

特性

• 製品名: MEWO, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 93082609, human skin, Fibroblast, 93082609
• 由来生物: human skin
• 包装: tube of 5 μg 93082609-DNA-5UG; pkg of vial of cells 93082609-1VL
• 成長モード: Adherent
• 核型: Not specified
• 形態: Fibroblast
• 製品: Melanin
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human malignant melanoma

細胞株の説明

Granular fibroblasts. Derived from a human melanoma by Prof. C Grose (1978). The cells support growth of varicella-zoster virus (VZV) isolates at 36°C although viral growth is optimal at 32°C.

アプリケーション

Virus studies

培地

EMEM（EBSS） + 2 mMグルタミン + 1％非必須アミノ酸（NEAA） + 10％ウシ胎児血清（FBS）

継代と培養方法

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA (0.5% trypsin in 0.53mM EDTA in modified Puck′s saline A); 5% CO₂; 37°C.

その他情報

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