SCL 4.1/F7

93031204, rat nerve (sheath), Flattened or crescent shaped

• UNSPSCコード: 41106514

特性

• product name: SCL 4.1/F7, 93031204
• 由来生物: rat nerve (sheath)
• 成長モード: Adherent
• 核型: 42,XY / 43,XY+7
• 形態: Flattened or crescent shaped
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Rat Schwann cell

細胞株の説明

This cell line was derived from neonatal Wistar rat Schwann cells being cultured for several months with intermittent exposure to the mitogen, cholera toxin and infrequent passaging to avoid premature transformation. After cloning one of these cultures by limiting dilution, the line SCL4.1/F7 was established growing without mitrogen. The cells display a subratum adhesion which is calcium-dependent in the millimolar range and pronounced contact-inhibition of growth. Confluent or subconfluent cultures readily cease proliferation and change to a differentiated (stellate / bipolar) morphology secreting an autocrine antiproliferative factor. Cells have been eradicated from mycoplasma at ECACC. With increasing passage cells have been shown to undergo transformation. It is recommended to growth-arrest cells for a few days before subculture to avoid rapid transformation.

培地

Ham′s F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

継代と培養方法

Split semi-confluent cultures 1:4 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C Saturation density is 10,000 cells/cm².

その他情報

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