A2058

91100402, human skin (lymph node metastasis), Epithelial

• 別名: A2058 Cell Line
• UNSPSCコード: 41106514

特性

• product name: A2058, 91100402
• 由来生物: human skin (lymph node metastasis)
• 成長モード: Adherent
• 核型: Not specified
• 形態: Epithelial
• 製品: Not specified
• 受容体: Nerve growth factor (NGF), Laminin
• テクニック: cell culture | mammalian: suitable
• 関連疾患: metastasis
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human caucasian metastatic melanoma

細胞株の説明

This cell line has been isolated from a lymph node metastasis from a 43 year old male patient with malignant melanoma. Due to their highly invasive properties, the cells can be used as a source of cellular invasion associated proteins e.g. collagen type IV collagenase, tissue inhibitor of metalloproteinase-2 and autocrine motility factor.

培地

EMEM（EBSS） + 2 mMグルタミン + 1％非必須アミノ酸（NEAA） + 10％ウシ胎児血清（FBS）

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

その他情報

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