RAW 264.7 Cell Line murine

91062702, mouse blood, Macrophage

• 別名: RAW264 7 Cells, RAW264.7 Cells
• UNSPSCコード: 41106514

特性

• 製品名: RAW 264.7 Cell Line murine, Macrophage from blood, 91062702
• 由来生物: mouse blood
• 成長モード: Semi-adherent
• 核型: Not specified
• 形態: Macrophage
• 製品: Lysozyme
• 受容体: Immunoglobulin, complement
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Mouse monocyte macrophage

細胞株の説明

Established from an ascites of a tumour induced in a male mouse by intraperitoneal injection of Abselon Leukaemia Virus (A-MuLV). Cells will pinocytose neutral red and phagocytose zymosan. Cells capable of antibody dependent lysis of sheep erythrocytes and tumour targets. Growth inhibited by LPS.

アプリケーション

Metabolic studies

RAW 264.7 cell line has been used to study the anti-inflammatory effects of pristimerin. It has also been used to test the internalization and intracellular survival of Listeria monocytogenes.

培地

DMEM + 2mM Glutamine + 10% FBS / FCS (FBS).

継代と培養方法

Split sub-confluent cultures (70-80%) 1:2 to 1:8 i.e. seeding at 2-4x10,000 cells/cm²; 5% CO₂; 37°C. Remove the cells mechanically. Cells are semi-adherent, i.e. some cells grow in suspension, some loosely attach to the surface and others flattened out and attached to the flask. Cells should not be allowed to overgrow and become confluent as this can lead to loss of the flattened adherent cell characteristic.

その他情報

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