FRTL-5

91030711, rat thyroid, Not specified

• UNSPSCコード: 41106514

特性

• product name: FRTL-5, 91030711
• 由来生物: rat thyroid
• 成長モード: Adherent aggregates
• 核型: Not specified
• 形態: Not specified
• 製品: Thyroglobulin
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Rat thyroid

細胞株の説明

This thyroid cell line has been established from normal thyroid glands from 5 to 6-week-old Fischer rats. FRTL-5 is a clonal line of the continuously cultured FRTL cells derived by successive clonal isolation. The cells are characterised by maintaining highly differentiated thyroid features such as secretion of thyroglobulin and concentration of iodide. FRTL-5 cells have been used as assays measuring the stimulatory activity of human autoimmune immunoglobulins from patients with Graves disease. Tend to grow in three-dimensional structures, monolayer not formed.

アプリケーション

Study of thyroid cells, hormone dependency and secretion.

培地

Coon′s modified Ham′s F12 medium (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol) + 2mM Glutamine + 10μg/ml Insulin + 10nM Hydrocortisone + 5μg/ml Transferrin + 10ng/ml gly-his-lys acetate + 10ng/ml somatostatin + 5% Foetal Bovine Serum (FBS) + 10mU/ml TSH. As an alternative to Coon′s modified Ham′s F12, it is possible to use Kaign′s modified Ham′s F12 supplemented with 45mg/L Ascorbic acid and 18mg/L Inositol.

継代と培養方法

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

その他情報

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