L-2

90112811, rat lung, Epithelial

• UNSPSCコード: 41106514

特性

• product name: L-2, 90112811
• 由来生物: rat lung
• 成長モード: Adherent
• 核型: Modal no. 77
• 形態: Epithelial
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Rat lung

細胞株の説明

Derived from normal rat lung tissue. The cells possess elevated levels of specific enzymes involved in the synthesis of phosphatidyl choline. Osmiophilic lamellar inclusions are observed in cell pellets from mass culture.

培地

Coon′s modified Ham′s F12 or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 to 1:4 i.e. seeding at 3-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

その他情報

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