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product name
PC-3 Cell Line human, 90112714, Caucasian prostate adenocarcinoma
由来生物
human prostate
成長モード
Adherent
核型
2n = 46, triploid, modal no. 62
形態
Epithelial
製品
Not specified
受容体
Not specified
テクニック
cell culture | mammalian: suitable
関連疾患
cancer
輸送温度
dry ice
保管温度
−196°C
細胞株の由来
Human Caucasian prostate adenocarcinoma
細胞株の説明
Established from a grade 4 prostatic adenocarcinoma from a 62 year old male Caucasian. The cells grow in agar and produce tumours in nude mice. Exhibit low acid phosphatase and testosterone-5-α reductase activity. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.
アプリケーション
発がん性の研究
PC-3 cell line has been used to study the antiproliferative effects of F. mughlea Pesmen on cancer cells. It has also been used to study the effects of palmitoylcarnitine (palcar) on the gene expression and pro-inflammatory pathways in prostate cancer.
DNAプロファイル
STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 11
D16S539: 11
D5S818: 13
D7S820: 8,11
THO1: 6,7
TPOX: 8,9
vWA: 17
CSF1PO: 11
D13S317: 11
D16S539: 11
D5S818: 13
D7S820: 8,11
THO1: 6,7
TPOX: 8,9
vWA: 17
培地
Coons Modified Ham’s F12 + 2mM Glutamine + 7% Foetal Bovine Serum (FBS).OR Kaign’s modified Ham’s F12 + 45mg/L ascorbic acid + 18 mg/L Inositol + 2mM Glutamine + 7% Foetal Bovine Serum (FBS).
継代と培養方法
Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Medium change every 5 days. The initial subculture interval after cells are thawed may be longer than 7-9 days.
その他情報
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試験成績書(COA)
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