MCA-RH 7777

90021504, rat liver, Hepatocyte

• UNSPSCコード: 41106514

特性

• product name: MCA-RH 7777, 90021504
• 由来生物: rat liver
• 成長モード: Adherent
• 核型: Not specified
• 形態: Hepatocyte
• 製品: Alpha-foetoprotein
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Rat Buffalo hepatoma

細胞株の説明

Derived from the Morris hepatoma 7777 (obtained from a male Buffalo strain rat). The original tumour was induced with N-2 fluorenylphthalamic acid. Particularly interesting for oncogeny studies since the cells produce α-fetoprotein (AFP).

アプリケーション

Tumourigenicity and oncogeny studies

培地

Swimm′s S-77 + 4mM Glutamine + 20% Horse Serum (HS) + 5% Foetal Bovine Serum (FBS) or EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 20% Foetal Bovine Serum (FBS).

継代と培養方法

Split semi-confluent cultures 1:2 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. NB: Cells are shed from the monolayer before confluency is achieved.

その他情報

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