153BR

90011808, human skin, Fibroblast

• UNSPSCコード: 41106514

特性

• product name: 153BR, 90011808
• 由来生物: human skin
• 成長モード: Adherent
• 核型: Not specified
• 形態: Fibroblast
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human skin fibroblast

細胞株の説明

Established from a biopsy of a normal 56 year old male. The cells have been used in radiation survival studies. EBV-transformed lymphocytes designated LB185 (Sigma Catalogue number. 98062318) have been isolated from the same individual. This has been confirmed by STR profiling at ECACC.

アプリケーション

DNA repair studies

DNAプロファイル

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11
D16S539: 9
D5S818: 10,13
D7S820: 11
THO1: 8,9
TPOX: 8,10
vWA: 17,18

培地

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS).

継代と培養方法

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

その他情報

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