Nthy-ori 3-1 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 90011609, human thyroid (follicular epithelial), Epithelial

• 別名: HTori-3.1 Cells, Nthy-ori 3.1 Cells
• UNSPSCコード: 41106514

特性

• 由来生物: human thyroid (follicular epithelial)
• 包装: tube of 5 μg 90011609-DNA-5UG; pkg of vial of cells 90011609-1VL
• 成長モード: Adherent
• 核型: Not specified
• 形態: Epithelial
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human thyroid follicular epithelial

細胞株の説明

Normal human primary thyroid follicular epithelial cells were transfected with a plasmid containing an origin-defective SV40 genome (SV-ori) to immortalise them. SV40 DNA was able to immortalise normal thyroid epithelial cells with the retention of several different functions.

アプリケーション

Nthy-ori 3-1 human cell Line has been used for transfection and virus studies. It is also used for the study of control and growth in human thyroid. The cell line has been used for microRNA (miRNA) expression profiling.

培地

RPMI 1640 + 2mM グルタミン + 10％ ウシ胎児血清（FBS）

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

その他情報

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