Sf9 Cell Line from Spodoptera frugiperda pupal ovarian tissue

89070101, insect ovary, Spherical

• 別名: SF-9 Cells
• UNSPSCコード: 41106514

特性

• 製品名: Sf9 Cell Line from Spodoptera frugiperda pupal ovarian tissue, 89070101
• 由来生物: insect ovary
• 成長モード: Adherent
• 核型: Not specified
• 形態: Spherical
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Spodoptera frugiperda pupal ovarian tissue

細胞株の説明

Derived from pupal ovarian tissue of spodoptera frugiperda. The cells are highly susceptible to Baculovirus infection and are used in the production of protein products genetically manipulated into Baculovirus vector systems.

アプリケーション

Sf9 cell line has been used for Baculovirus viral studies. It has also been used for in vitro kynurenine-3-monooxygenase (KMO) inhibition assay.

培地

TC100 + 2mM Glutamine. (The medium can be supplemented with 10% Foetal Bovine Serum (FBS) if required).

継代と培養方法

Maintain cultures between 3x100,000 to 1x1,000,000 cells/ml; 27°C. Gently resuspend cells in old culture medium by dislodging the cells by pipetting, or gentle agitation, and resuspend in fresh medium. Cells should stick to the culture flask. Freeze in 9% DMSO/91% FBS. Cultures may take up to 10 days to recover normal growth characteristics following cryopreservation and resuscitation.

その他情報

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