OX-35 Cell Line from murine myeloma

clone 5, Reactivity: Anti- CD4, rat, 89041184

• UNSPSCコード: 41106514

特性

• 由来生物: Mouse x Mouse Hybridoma
• クローン: 5, monoclonal
• 包装: tube of 5 μg 89041184-DNA-5UG; pkg of vial of cells 89041184-1VL
• ImmunoDon: BALB/c mouse spleen
• 骨髄腫: NS0/1
• 反応性: Anti-CD4, rat
• テクニック: cell culture | mammalian: suitable
• アイソタイプ: IgG2a
• 輸送温度: dry ice

詳細

細胞株の由来

Mouse NS0/U x Mouse BALB/c spleen

細胞株の説明

The antibody is directed against the CD4 antigen on rat T cells and macrophages. The specificity is the same as that of W3/25, though their binding is non-competitive. OX-35 will compete with OX-36.

免疫原

T blasts prepared in mixed lymphocyte reactions with purified rat T helper cells against irradiated spleen

培地

RPMI 1640 + 2mM グルタミン + 10％ ウシ胎児血清（FBS）

継代と培養方法

Seed at 5x10,000 viable cells/ml. Subculture when cells reach 5x100,000-1x1,000,000 cells/ml (usually after 3-4 days); 5% CO₂; 37°C.

その他情報

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免責事項

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.