SW 48

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 89012702, human colon, Epithelial

• UNSPSCコード: 41106514

特性

• 由来生物: human colon
• 包装: tube of 5 μg 89012702-DNA-5UG; pkg of vial of cells 89012702-1VL
• 成長モード: Adherent
• 核型: 2n = 46
• 形態: Epithelial
• 製品: Carcinoembryonic antigen (CEA)
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human colon adenocarcinoma

細胞株の説明

The cells produce a low level of CEA: 0.6ng/1,000,000 cells in 10 days. Cells are tumorigenic in nude mice. Established from a grade IV ulcerating tumour encircling the bowel of an 82 year old Caucasian female. HLA = A32, A33, B27, B35

アプリケーション

発がん性の研究

DNAプロファイル

STR-PCR Data: Amelogenin: X
CSF1PO: 9,10
D13S317: 11,12
D16S539: 11,13
D5S818: 10,14
D7S820: 9,10
THO1: 6,9.3
TPOX: 8
vWA: 18,20

培地

L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; No CO₂; 37°C.

その他情報

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