HL60 Ast.3

88120801, human blood, Lymphoblastoid

• UNSPSCコード: 41106514

特性

• product name: HL60 Ast.3, 88120801
• 由来生物: human blood
• 成長モード: Suspension
• 核型: Modal no. = 93, Hypertetraploid
• 形態: Lymphoblastoid
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human Caucasian promyelocytic leukaemia

細胞株の説明

Variant of the promyelocytic cell line HL60 isolated after treating cells with α-particle radiation and then cloned in medium containing 1.25% DMSO. Cells are capable of differentiating into neutrophils by induction with 1.75% DMSO.

アプリケーション

Differentiation studies

DNAプロファイル

STR-PCR Data: Amelogenin: X
CSF1PO: 14
D13S317: 8,11
D16S539: 11
D5S818: 12
D7S820: 11,12
THO1: 7
TPOX: 8,11
vWA: 16

培地

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 1.25% Dimethyl Sulphoxide (DMSO).

継代と培養方法

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. Do not allow the culture medium to become acidic or the cell line will differentiate.

その他情報

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