ML-1

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 88113007, human blood, Lymphoblastoid

• UNSPSCコード: 41106514

特性

• 由来生物: human blood
• 包装: tube of 5 μg 88113007-DNA-5UG; pkg of vial of cells 88113007-1VL
• 成長モード: Suspension
• 核型: Not specified
• 形態: Lymphoblastoid
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human acute myeloblastic leukaemia

細胞株の説明

ML-1 is one of three celll lines isolated in 1978 from the peripheral blood of a 24 year old male patient with acute myeloblastic leukaemia. The cells can convert to more mature cells by the use of DMSO. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

アプリケーション

ML-1 has been used to compare the cell response to mafosfamide cyclohexylamine salt, 4-hydro-peroxy-cyclophosphamide and β-D-glucose-isophosphoramide mustard, which are oxazaphosphorine agents.

DNAプロファイル

STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 9,12
D16S539: 9,12
D5S818: 12
D7S820: 9,11
THO1: 7,9.3
TPOX: 8,10
vWA: 16

培地

RPMI 1640 (R0883) + 2mM L-Glutamine (G7513) + 10% FBS (F2442)

継代と培養方法

Maintain cultures between 2-9 x100,000 cells/ml; 5% CO₂; 37°C. Freeze in 10% DMSO + 90% FBS. Immediately after resuscitation, pellet cells by centrifugation at 150 x g for 5 minutes and resuspend the cell pellet in fresh medium. This is to remove the presence of DMSO which may cause differentiation of the cells if allowed to remain.

その他情報

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