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製品名
NCTC clone 1469, 88111403
由来生物
mouse liver
成長モード
Adherent
核型
Not specified
形態
Epithelial
製品
Not specified
受容体
Not specified
テクニック
cell culture | mammalian: suitable
輸送温度
dry ice
保管温度
−196°C
細胞株の由来
Mouse C3H/An liver
細胞株の説明
Derived from mouse liver cell line NCTC 721 which was obtained from normal liver of a 2 day old C3H/An mouse. The cells have been used in biochemical and nutritional studies. The cells are susceptible to VSV (Indiana strain).
アプリケーション
Cell suspension systems. Biochemical, nutritional and virus studies. VSV(Indiana)
培地
NCTC 135 + 2mM Glutamine + 10% Horse Serum (HS)
継代と培養方法
Split sub-confluent cultures (70-80%) 1:2 to 1:4 using only mechanical techniques, such as scraper, do not use trypsin, 5-10CO2; 37°C. Change media every 3 days. Freeze in 10% glycerol/90% FBS. Cells are shed into the medium giving it a turbid appearan
その他情報
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