T84 Cell Line human

88021101, human colon (lung metastasis), Epithelial

• 別名: T-84 Cells
• UNSPSCコード: 41106514

特性

• product name: T84 Cell Line human, 88021101
• 由来生物: human colon (lung metastasis)
• 詳細: Human colon carcinoma
• 成長モード: Adherent
• 核型: 2n = 46, modal no. 56
• 形態: Epithelial
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: metastasis
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human colon carcinoma

細胞株の説明

Derived from a lung metastasis of colon carcinoma in a 72 year old male. Tumour tissue was inoculated sub-cutaneously and serially transplanted in BALB/c nude mice and subsequently established in in vitro culture. The histological characteristics of the tumour were maintained throughout the transplantation procedure. They have receptors for a range of peptide hormones and neurotransmitters. The cells grow as monolayers, exhibit tight junctions and desmosomes between adjacent cells.

アプリケーション

Human T84 cell line has been used for comparative genome analysis of Salmonella enterica serovar Typhimurium definitive type 2 (DT2) isolate 94-213 and S. Typhimurium isolates SL1344, DT104 and D23580. The ability of these isolates to invade culture medium has also been studied. It has also been used to construct and study the suitability of an in vitro model to study chemotherapy-induced mucosal damage.

Tumourigenicity, neurotransmitter and hormone studies

培地

Ham′s F12 + DMEM (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

継代と培養方法

Split sub-confluent cultures (70-80%) 1:2 - 1:4 i.e. seeding at 1-3 x 10,000 cells / cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Cells grow slowly and may not form a complete monolayer, maintain at high density with a minimum of 25% confluency.

その他情報

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.