BB

87101201, fish trunk, Fibroblast

• UNSPSCコード: 41106514

特性

• product name: BB, 87101201
• 由来生物: fish trunk
• 成長モード: Adherent
• 核型: 2n = 60
• 形態: Fibroblast
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Fish Brown Bullhead posterior trunk tissue

細胞株の説明

Derived from the trunk tissue (excluding fins) of a 2 year old Brown Bullhead fish. These cells will tolerate a temperature range of 4-34°C. The optimal temperature range for cell proliferation is 25-30°C. BB is susceptible to infectious pancreatic necrosis virus and channel catfish virus.

アプリケーション

Virus studies: Infectious pancreatic necrotis virus and Channel catfish virus and channel catfish virus

培地

EMEM（EBSS） + 2 mMグルタミン + 1％非必須アミノ酸（NEAA） + 10％ウシ胎児血清（FBS）

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 using 0.25% trypsin/EDTA; 5% CO₂; 23°C. Note: The cells do not grow well on glass. Fish cell lines detach easily during transit if the culture is too young. After resuscitation split cells 1:3 and culture for a

その他情報

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