6-23 (clone 6)

87042206, rat thyroid, Epithelial

• UNSPSCコード: 41106514

特性

• product name: 6-23 (clone 6), 87042206
• 由来生物: rat thyroid
• 成長モード: Adherent
• 核型: Not specified
• 形態: Epithelial
• 製品: Neurotensin and calcitonin
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Rat medullary thyroid carcinoma

細胞株の説明

Derived from the non-clonal line rMTC 6-23 originally established from a transplantable WAG/RIJ rat medullary thyroid carcinoma. The cell line synthesises and secretes both neurotensin and calcitonin. Its clonal derivation demonstrates that a population derived from a single type of C-cell has the capability of producing both peptides. Secretion of these peptides has been shown to be stimulated by calcium, KC1, glucagon and catecholamines.

アプリケーション

Hormone studies

培地

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). Cells need to be grown on flasks coated with matrigel or any other attachment factor

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. For cell attachment culture vessels need to be coated with collagen type IV e.g. Sigma product C-5533. Soak in a sol

その他情報

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.