AtT20

87021902, mouse brain, Small round cells growing as large aggregates

• UNSPSCコード: 41106514

特性

• product name: AtT20, 87021902
• 由来生物: mouse brain
• 成長モード: Aggregates in suspension
• 核型: Diploid
• 形態: Small round cells growing as large aggregates
• 製品: Adrenocorticotropic hormone (ACTH)
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Mouse LAF1 pituitary gland tumour

細胞株の説明

Derived after alternate passage of LAF1 mouse pituitary tumour cells as tumours in animals and in cell culture. Cells produce adrenocorticotropic hormone (ACTH) at approximately the rate of 1000mU/mg of cell protein per week.

アプリケーション

Hormone studies

培地

Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS). Cells grow in large aggregates.

継代と培養方法

Allow clusters to settle and remove all but 5 to 10 ml of medium. Gently transfer the clusters to new flasks and add fresh medium. A subcultivation ratio of 1:2 is recommended. Cells grow in large viable clusters making counting difficult. A seeding densi

その他情報

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