RPMI 8226

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 87012702, human blood, Not specified

• 別名: RPMI8226 Cells, Roswell Park Memorial Institute 8226 Cells
• UNSPSCコード: 41106514

特性

• 由来生物: human blood
• 包装: tube of 5 μg 87012702-DNA-5UG; pkg of vial of cells 87012702-1VL
• 成長モード: Suspension
• 核型: Not specified
• 形態: Not specified
• 製品: Immunoglobulin G (Ig) lambda light chain
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human myeloma

細胞株の説明

Derived from the peripheral blood of a 61 year old male with multiple myeloma. The cells produce and secrete Ig lambda light chain.

アプリケーション

RPMI 8226 has been used:

• to grow human myeloma cell lines
• to evaluate the effect of 1-aryl-2-methylthioimidazolines (IIV) on the viability of RPMI 8226 cells
• to measure cytokine production by bone marrow stromal cells (BMSCs) after contact with the RPMI 8226 cell line

DNAプロファイル

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 12
D13S317: 11
D16S539: 9
D5S818: 11,13
D7S820: 9,10
THO1: 8
TPOX: 8,11
vWA: 16,18

培地

RPMI 1640 + 2mM グルタミン + 10％ ウシ胎児血清（FBS）

継代と培養方法

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. After resuscitation cell debris will be present in culture. This will decrease with subsequent subculture.

その他情報

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