GH3

87012603, rat brain, Epithelial

• UNSPSCコード: 41106514

特性

• product name: GH3, 87012603
• 由来生物: rat brain
• 成長モード: Adherent
• 核型: Hyperdiploid, modal no. 60
• 形態: Epithelial
• 製品: Growth hormone, prolactin
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Rat Wistar-Furth pituitary tumour

細胞株の説明

Derived from a 7 month female Wistar-Furth rat. GH3 cells produce growth hormone faster than the GH1 cell line and also produce prolactin. Hydrocortisone stimulates growth hormone production and inhibits prolactin production. Studies have shown that cells are capable of adaptation to suspension culture using MEM.

アプリケーション

Hormone studies

培地

Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).

継代と培養方法

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Upon thawing, the time taken before the first subculture can be performed may be extended (10-15 days). To aid attatchment, flask

その他情報

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