CRFK (BVD Ag negative)

86093002, feline kidney, Epithelial

• UNSPSCコード: 41106514

特性

• product name: CRFK (BVD Ag negative), 86093002
• 由来生物: feline kidney
• 成長モード: Adherent
• 核型: Hypodiploid, modal no 36
• 形態: Epithelial
• 製品: Carcinoembryonic antigen (CEA)
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Cat kidney

細胞株の説明

CRFK cells were isolated from the cortical portion of the kidneys of a 10-12 week old normal female domestic cat. CRFK are extensively used in feline virus research. Susceptible to herpesvirus and feline picornaviruses producing CPE. BVD Ag negative.

アプリケーション

Crandell feline kidney cells (CRFK) (BVD Ag negative) have been used in viral studies.

培地

EMEM（EBSS） + 2 mMグルタミン + 1％非必須アミノ酸（NEAA） + 10％ウシ胎児血清（FBS）

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

その他情報

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