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製品名
BC3H1, 86093001
由来生物
mouse brain
包装
tube of 5 μg 86093001-DNA-5UG
pkg of vial of cells 86093001-1VL
成長モード
Adherent
核型
Not specified
形態
Fibroblast
製品
Acetylcholine
受容体
Not specified
テクニック
cell culture | mammalian: suitable
関連疾患
cancer
輸送温度
dry ice
保管温度
−196°C
細胞株の由来
Mouse brain tumour
細胞株の説明
Established in 1974 from a mouse brain neoplasm tumour induced by nitrosoethylurea, possess many properties characteristic of smooth muscle. Since then it has been suggested that BC3H1 is a skeletal line of ectodermal origin that is defective for commitment to terminal differentiation. The two distinct morphologies typical of this cell line are large spheroid cells and smooth-muscle-like cells. The fusiform is the more common.
アプリケーション
Acetylcholine receptor studies.
培地
DMEM + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).
継代と培養方法
Spilt confluent cultures 1:3 to 1:10 i.e. seeding at 1-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
その他情報
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