CL 21

86080602, Human x Mouse Hybridoma unknown/unspecified, Fibroblast-like

• UNSPSCコード: 41106514

特性

• product name: CL 21, 86080602
• 由来生物: Human x Mouse Hybridoma unknown/unspecified
• 成長モード: Adherent
• 核型: Not specified
• 形態: Fibroblast-like
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Mouse / Human hybrid, SV-40 transformed

細胞株の説明

The cell line CL21 has been established by hybridisation of C57 BL mouse peritoneal macrophages with SV40 transformed human skin fibroblasts. The human cells were derived from a patient with Lesch-Nyhan syndrome and are deficient in hypoxanthine guanine phosphoribosyltransferase (HGPRT). They have been reported to contain the human chromosome 7 and could also contain subchromosomal fragments of chromosome 17.

アプリケーション

Somatic cell hybrid mapping

培地

DMEM + 2mM グルタミン + 10％ ウシ胎児血清（FBS）

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

その他情報

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