A7r5 Cell Line from rat

86050803, rat muscle, Fibroblast

• UNSPSCコード: 41106514

特性

• product name: A7r5 Cell Line from rat, 86050803
• 由来生物: rat muscle
• 詳細: Rat BDIX smooth muscle embryonic aorta
• 成長モード: Adherent
• 核型: Not specified
• 形態: Fibroblast
• 製品: Muscle-type isoenzymes
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Rat BDIX smooth muscle embryonic aorta

細胞株の説明

Flat ribbon-like cells differentiating to parallel arrays of spindle shaped cells. Derived from the thoracic aorta of an embryonic BDIX rat. As cells achieve the stationary phase there is an increase in myokinase and creatine phosphokinase (CPK) activity. After cessation of cell division a muscle type CPK isoenzyme is synthesised.

アプリケーション

A7r5 Cell Line from rat has been used to study the effect of angiotensin II on vascular oxidative stress. It has been used in PLA2 inhibitory study to understand the role of PLA2 on formation of lipid droplets.

Biochemical and biophysical studies

培地

DMEM + 2mM グルタミン + 10％ ウシ胎児血清（FBS）

継代と培養方法

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. After resuscitation cells may take up to 7 days before a monolayer forms and growth can be very slow in general. Cells also grow in clumps.

その他情報

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