P3HR-1

86010701, human lymph, Lymphoblast

• UNSPSCコード: 41106514

特性

• product name: P3HR-1, 86010701
• 由来生物: human lymph
• 成長モード: Suspension
• 核型: 2n = 46, hyperdiploid
• 形態: Lymphoblast
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human Burkitt′s Lymphoma

細胞株の説明

Clonally-derived sub-line of JIYOYE (Sigma Catalogue number. 88071302) containing high levels of EBV. Has been successfully hybridised with the human lymphoid cell line K562 to form the hybrid PUTKO-1. ACDP class II pathogen and should be handled under laboratory containment level 2. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

DNAプロファイル

STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 12
D16S539: 10,11
D5S818: 12
D7S820: 8,10
THO1: 7,9
TPOX: 6,8
vWA: 15,19

培地

RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

継代と培養方法

3～9×100,000細胞/mL、5％ CO₂、37℃の状態を維持して培養してください。

その他情報

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