WiDr

85111501, human colon, Epithelial

• UNSPSCコード: 41106514

特性

• 製品名: WiDr, 85111501
• 由来生物: human colon
• 成長モード: Adherent
• 核型: 2n = 46, hypertriploid
• 形態: Epithelial
• 製品: Carcinoembryonic antigen (CEA)
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human colon adenocarcinoma

細胞株の説明

Although originally described as a colon adenocarcinoma line established from a 78 year old female, DNA fingerprinting has shown this line to be a derivative of HT-29. HT29 was isolated from a primary colon adenocarcinoma in a 44 year old Caucasian female. The cells produce carcinoembryonic antigen and are tumorigenic in xenogeneic animals. They have been used in studies of anti-tumour agents.

アプリケーション

Carcinogenesis and tumourigenicity studies and anti-tumour testing

DNAプロファイル

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 11,12
D16S539: 11,12
D5S818: 11,12
D7S820: 10
THO1: 6,9
TPOX: 8,9
vWA: 17,19 STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 11,1

培地

EMEM（EBSS） + 2 mMグルタミン + 1％非必須アミノ酸（NEAA） + 10％ウシ胎児血清（FBS）

継代と培養方法

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 1-2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

その他情報

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