PLC/PRF/5

85061113, human liver, epithelial

• 別名: Alexander cell line
• UNSPSCコード: 41106514

特性

• 製品名: PLC/PRF/5, from human liver, 85061113
• 由来生物: human liver
• 包装: tube of 5 μg 85061113-DNA-5UG; pkg of vial of cells 85061113-1VL
• 成長モード: Adherent
• 核型: Not specified
• 形態: epithelial
• 製品: Hepatitis B Surface Antigen (HBSAg)
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の説明

Alexander cell line which produces Hepatitis B antigen. The virus genome may be inducible in selective media. The cells should be handled under laboratory containment level 2. Expresses c-abl, c-fes, c-fms, c-myc, c-ha-ras and c-sis oncogenes. However there is currently no evidence that this cell line produces infectious Hepatitis B virus.

DNAプロファイル

STR-PCR Data:

Amelogenin: X
CSF1PO: 10
D13S317: 11,12
D16S539: 13
D5S818: 12
D7S820: 9,11
THO1: 7,8
TPOX: 8
vWA: 15,16

培地

DMEM + 2mM Glutamine + 10% fetal bovine serum (FBS).

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 to 1:6; i.e., seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

その他情報

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