MRC-5 SV1 TG2

85042502, human lung (fetal), Fibroblast

• UNSPSCコード: 41106514

特性

• 製品名: MRC-5 SV1 TG2, 85042502
• 由来生物: human lung (fetal)
• 成長モード: Adherent
• 核型: Modal no. = 60-80 hperdiploid
• 形態: Fibroblast
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human foetal lung, SV40 transformed

細胞株の説明

A clonal derivative of an SV40 transformed permanent line of MRC-5. Selection was made using X-ray induction in the presence of 2μg/ml thioguanine. The cells are HAT sensitive.

DNAプロファイル

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11,14
D16S539: 11
D5S818: 11,12
D7S820: 10
THO1: 8
TPOX: 8
vWA: 15

培地

EMEM（EBSS） + 2 mMグルタミン + 1％非必須アミノ酸（NEAA） + 10％ウシ胎児血清（FBS）

継代と培養方法

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 1-2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

その他情報

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