C57/B1

85011438, mouse skin, Epithelial

• UNSPSCコード: 41106514

特性

• product name: C57/B1, 85011438
• 由来生物: mouse skin
• 成長モード: Adherent
• 核型: Not specified
• 形態: Epithelial
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Mouse C57BL/6J melanoma epithelial

細胞株の説明

Spontaneous tumour of C57 black/6J mouse originally obtained from Dr Parrish of Australian National University, Canberra. Rendered HGPRT- by selection in 20μg/ml 6-thioguanine.

アプリケーション

発がん性の研究

培地

EMEM（EBSS） + 2 mMグルタミン + 1％非必須アミノ酸（NEAA） + 10％ウシ胎児血清（FBS）

継代と培養方法

Split sub-confluent cultures (70-80%) at a maximum dilution of 1:10 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Monolayers will detach if monolayer becomes over-confluent.

その他情報

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