Hep G2 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA. 85011430, human liver, Epithelial

• 別名: HepG2 Cells
• UNSPSCコード: 41106514

特性

• 由来生物: human liver
• 包装: tube of 5 μg 85011430-DNA-5UG; vial of cells 85011430-1VL
• 成長モード: Adherent
• 核型: Modal no. 55
• 形態: Epithelial
• 製品: Prothrombin, antithromin III, alpha-foetoprotein complement, C3 and C4 activator, fibrinogen
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human Caucasian hepatocyte carcinoma

細胞株の説明

The Hep G2 cell line has been isolated from a liver biopsy of a male Caucasian aged 15 years, with a well differentiated hepatocellular carcinoma. The cells secrete a variety of major plasma proteins e.g. albumin, α2-macroglobulin, α 1-antitrypsin, transferrin and plasminogen. They have been grown successfully in large scale cultivation systems. Hepatitis B virus surface antigens have not been detected. The cells will respond to stimulation with human growth hormone.

アプリケーション

Hep G2 cell line human has been used to study the cytotoxicity of carbon monoxide-releasing molecules and organic solvents on HepG2 cells and to evaluate the antiproliferative activity of aqueous extracellular polysaccharides (AEPS) for HepG2 using the MTT colorimetric assay.

Study of hepatocyte function and specific protein expression

包装

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

DNAプロファイル

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 9,13
D16S539: 12,13
D5S818: 11,12
D7S820: 10
THO1: 9
TPOX: 8,9
vWA: 17

培地

EMEM (EBSS)+ 2mM Glutamine + 1% Non Essential Amino Acids NEAA) + 10% Foetal Bovine Serum FBS / FCS.

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Cells might take 6 days to form a confluent monolayer without media change if seeded at 1:4. Until then growth occurs mainly in islands.

その他情報

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