B82

85011408, mouse adipose tissue, Fibroblast

• UNSPSCコード: 41106514

特性

• product name: B82, 85011408
• 由来生物: mouse adipose tissue
• 成長モード: Adherent
• 核型: 2n = 51
• 形態: Fibroblast
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Mouse C3H/An sc areolar and adipose tissue

細胞株の説明

Derivative of NCTC clone 929 (Sigma Catalogue number. 88102702) obtained by progressive selection in BUdR, has a cloning efficiency of 70-80%.

アプリケーション

Chromosome analysis and hybridisation studies

培地

EMEM（EBSS） + 2 mMグルタミン + 1％非必須アミノ酸（NEAA） + 10％ウシ胎児血清（FBS）

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

その他情報

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