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製品名
TK6 (IVGT), 13051501, from human spleen
由来生物
human spleen
詳細
Human lymphoblast, thymidine kinase heterozygote, mutagenesis assay, diploid human
包装
tube of 5 μg 13051501-DNA-5UG
pkg of vial of cells 13051501-1VL
成長モード
Suspension
形態
Small round cells in suspension
テクニック
cell culture | mammalian: suitable
輸送温度
dry ice
保管温度
−196°C
細胞株の説明
JCRBNo 1435, lot number 11032011. This line is a derivative of the WIL-2 lymphoblast cell line. The cells are heterozygous at the thymidine (TK) kinase locus and can be used to quantitatively detect forward mutations at 3 loci.
培地
RPMI 1640 (R0883) + 10% Horse Serum + 2mM L-Glutamine (G7513) + 1.8 mMolar Sodium Pyruvate(NaP) (S8636). Cryopreserve cells in culture medium with cells heat-inactivated 10% Horse Serum and 10% Dimethyl sulphoxide (DMSO)
継代と培養方法
Dilute cell in fresh medium at 0.1- 1.5 x 105 cells per ml.
その他情報
Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
If use of this culture results in a scientific publication, it should be cited in the publication as: TK6 (IVGT) (ECACC 13051501)
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