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製品名
TK6 (IVGT), from human spleen, 13051501
由来生物
human spleen
詳細
Human lymphoblast, thymidine kinase heterozygote, mutagenesis assay, diploid human
成長モード
Suspension
形態
Small round cells in suspension
テクニック
cell culture | mammalian: suitable
輸送温度
dry ice
保管温度
−196°C
細胞株の説明
JCRBNo 1435, lot number 11032011. This line is a derivative of the WIL-2 lymphoblast cell line. The cells are heterozygous at the thymidine (TK) kinase locus and can be used to quantitatively detect forward mutations at 3 loci.
培地
RPMI 1640 (R0883) + 10% Horse Serum + 2mM L-Glutamine (G7513) + 1.8 mMolar Sodium Pyruvate(NaP) (S8636). Cryopreserve cells in culture medium with cells heat-inactivated 10% Horse Serum and 10% Dimethyl sulphoxide (DMSO)
継代と培養方法
Dilute cell in fresh medium at 0.1- 1.5 x 105 cells per ml.
その他情報
Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
引用
If use of this culture results in a scientific publication, it should be cited in the publication as: TK6 (IVGT) (ECACC 13051501)
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