FM-78

from human skin, 13012420

• UNSPSCコード: 41106514

特性

• 由来生物: human skin
• 詳細: Human melanoma
• 成長モード: Adherent
• 形態: Fibroblastic
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の説明

FM-78 (ESTDAB-023) was established from a metastatic cutaneous malignant melanoma. The FM-78 cell line is one of more than 170 characterized melanoma cell lines collected during the ESTDAB project (European Searchable Tumour Line Database) part of the European Commission′s fifth framework infrastructures programme (contract no. QLRI-CT-2001-01325). ESTDAB contains information on HLA genotype and surface expression of HLA molecules, expression of tumour antigens, antigen processing capability, production of and response to cytokines and chemokines, apoptosis regulation and expression of adhesion/accessory molecules. Such information allows researchers to identify cells possessing specific parameters important for studies of immunity, immunogenetics, gene expression, metastasis, response to chemotherapy, and other tumour biological experimentation.

DNAプロファイル

STR-PCR Data:

Amelogenin: X
CSF1PO: 10,11
D13S317: 8,11
D16S539: 12
D5S818: 12
D7S820: 10
THO1: 9,9.3
TPOX: 8,11
vWA: 15,16

培地

RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS).

継代と培養方法

Split sub-confluent cultures (70-80%) seed new cultures at 2-4 x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Saturation density 10 x 10⁴ cells/cm².

その他情報

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