AE17 cell line from mouse lung

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 10092310, mouse lung, Cells are epithelial-like

• 別名: AE17 Cells
• UNSPSCコード: 41106514

特性

• 由来生物: mouse lung
• 詳細: Mouse malignant mesothelioma
• 包装: tube of 5 μg 10092310-DNA-5UG; pkg of vial of cells 10092310-1VL
• 成長モード: Adherent
• 核型: Not specified
• 形態: Cells are epithelial-like
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Mouse malignant mesothelioma

細胞株の説明

Mice (C57Black/6J strain female 6-8 weeks old) were exposed to crocidolite asbestos through intraperitoneal injection resulting in tumour development (both ascites and solid tumours). Cultures were established from malignant mesothelial cells obtained from ascites fluid. Cells are tumourigenic in syngeneic immunocompetent mice. Cell Bank Australia reference number: CBA0156

アプリケーション

Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods.

培地

RPMI1640 (with 2mM L-Glutamine+25mM HEPES) + 5% FCS

継代と培養方法

Optimal split ratio 1:16 - 1:32 (seeding density 0.6 x104 cells/cm²).Cells incubated at 37°C with 5% CO₂.Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min.

その他情報

HPA Culture Collections由来でSIGMAによって供給される培養物は研究の目的のみに使用できます。商業目的での細胞株の使用に関する質問は、その細胞株のデポジターに照会されます。一部の細胞株には、細胞株の供給の前に受給予定者によって物質移動契約書への記入を完了させるという要求など、さらなる特別な公開条件があります。詳しくはTerms & Conditions of Supplyをご覧ください。

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Depositor and originator: Richard Lake - University of Western Australia, Australia