BRIN-BG7

10020902, rat pancreas (islets), Epitheloid

• 別名: BRIN
• UNSPSCコード: 41106514

特性

• product name: BRIN-BG7, from rat pancreas(islets), 10020902
• 由来生物: rat pancreas (islets)
• 成長モード: Adherent
• 核型: Modal chromosome number 66 up to passage 50
• 形態: Epitheloid
• 製品: BRIN-BG7 is a stable, glucose-responsive insulin secreting beta cell line expressing insulin, glucokinase and IAPP as detected by immunocytochemistry. BRIN-BG7 cells express the GLUT2 glucose transporter.
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice

詳細

細胞株の由来

A rat pancreatic Beta cell: RINm5F hybrid cell line. Insulin-secreting cell line

DNAプロファイル

特記事項なし

培地

RPMI-1640 + 2 mM Glutamine + 10% FCS / FBS

継代と培養方法

Split sub-confluent cultures (70-80%) 1:8 to 1:10 , i.e., seeding at 2-4 x 10⁴ cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37 °C. Population doubling approx 20hrs. At confluence 10⁵ cells/cm² can be expected.

その他情報

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

免責事項

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.