T7

7021402, mouse lung, Epithelial

• UNSPSCコード: 41106514

特性

• product name: T7, 07021402
• 由来生物: mouse lung
• 成長モード: Adherent
• 核型: Not specified
• 形態: Epithelial
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Mouse Type-II lung epithelial cell

細胞株の説明

Fully differentiated Type-II alveolar epithelial cell line derived from transgenic H-2Kb-tsA58 mice capable of being passaged as an immortalised cloned cell line in culture. Cells will proliferate at 33 - 35°C in the presence of gamma-interferon, at 39°C in the absence of gamma-interferon cells will terminally differentiate. Supplied as frozen ampoules only.

培地

DMEM + 2mM Glutamine + 4% Foetal Bovine Serum (FBS) + 8 additional supplements - please see ′Additional Literature Report′ field below for full details. ECACC can supply an information sheet on the preparation of the supplements on request - contact ECAC

継代と培養方法

Split confluent cultures 1:5 to 1:10 i.e. seeding at 2-4x10,000 cells/cm² using trypsin/EDTA;10% CO₂; 33°C. Doubling time 48h

その他情報

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