C22 (Clara)

7021401, mouse lung, Epithelial

• UNSPSCコード: 41106514

特性

• 製品名: C22 (Clara), 07021401
• 由来生物: mouse lung
• 成長モード: Adherent
• 核型: Not specified
• 形態: Epithelial
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Mouse lung epithelial progenitor cell

細胞株の説明

A progenitor cell of the peripheral airway epithelium derived from transgenic H-2Kb-tsA58 mice capable of being passaged as an immortalised cloned cell line in culture. Cells will proliferate at 33°C in the presence of gamma-interferon, at 39°C in the absence of gamma-interferon cells will terminally differentiate. Supplied as frozen ampoules only.

培地

DMEM + 2 mM Glutamine + 4% Fetal Bovine Serum (FBS) + 8 supplements:

0.25 μg/ml Endothelin-1
0.01 μg/ml Interferon gamma
10 μg/ml Insulin
5 μg/ml Transferrin
7.5 μg/ml Endothelial Cell Growth Supplement (ECGS)
0.025 μg/ml Epidermal Growth Factor
0.36 μg/ml Hydrocortisone
0.02 μg/ml T3

For guidance on the supplements and preparation of the culture medium, ECACC can supply an information sheet on request - contact ECACC Technical Support.

継代と培養方法

Split confluent cultures 1:5 to 1:10 i.e. seeding at 2-4x10,000 cells/cm² using trypsin/EDTA;10% CO₂; 33°C. Doubling time 48h

その他情報

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